Plating Media: R medium + 2 uM metatopolin + 4, 1cm3 cubes Russet potatoe. Bleach 25 mins. in 0.525% chlorox bleach
Replating Media: R medium + 1, 1cm3 cube Russet potato
Number of Days between Plating and Replating: 61 days
Number of Days between Replating and Vernalization: 256 days
Number of Days between start and end of Vernalization: 120 days (4 months)
Date to Initiate Plating: Jan 17
Date to Replate: March 19
Date to start to Vernalize: Dec. 1
Results:
2010
Seedlings plated 11-05-09, replated on 01-0502010, started vernalization on 09-08-2010. Seedlings were 1/2 to 1 inches tall and the roots were hairy, 3-6 in number, and 3-4 inches long. Need to start plating earlier, Jan. 17, so vernalization process is not 6 months long. Some of the seedlings were sprayed with fung-onil to control the growth of fungus. Overall protocol produced very good results.